Chrysophanol Inhibits NALP3 Inflammasome Activation and Ameliorates Cerebral Ischemia/Reperfusion in Mice

Figure 4

The dynamic expression of NALP3 inflammasome and IL-1β during the natural inflammatory response induced by tMCAO. Western blot of dynamic expression ((a), (b), (c)). Bar graph illustrating the dynamic expression of NALP3, ASC, active caspase-1, and active IL-1β in brain tissues (d). Bar graph illustrating dynamic expression of NALP3, ASC, caspase-1, and IL-1β as determined by immunohistochemistry (e). Bar graph illustrating the mRNA dynamic expression of NALP3, ASC, caspase-1, and IL-1β (f). For NALP3, ^☆𝑃<0.05 versus Sham, ^★𝑃<0.05 versus Sham, 3 h, 6 h, 12 h, 48 h, and 72 h. For ASC, ^△𝑃<0.05 versus Sham. For active caspase-1 and active IL-1β, ^◊𝑃<0.05 and ^□𝑃<0.05 versus Sham, ^◆𝑃<0.05 and ^■𝑃<0.05 versus Sham, 3 h, 6 h, 12 h, and 72 h.

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