MicroRNA-146a Decreases High Glucose/Thrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression
Figure 6
The proinflammatory phenotypes of HG/thrombin-stimulated HAECs were attenuated by a miR-146a mimic. (a) Analysis using an inflammatory cytokine kit showed that the stimulatory effect of HG/thrombin on IL-8 and IL-6 protein expression in conditioned mediums was preserved in miR-control-transfected HAECs, while the stimulatory effect was attenuated in miR-146a mimic-transfected, HG/thrombin-stimulated HAECs. The images shown represent the results from 3 independent experiments. (b) The stimulatory effect of HG/thrombin on THP-1 adhesion to HAECs was preserved in miR-control-transfected HAECs, while the THP-1 adhesiveness was attenuated in miR-146a mimic-transfected, HG/thrombin-stimulated HAECs to 34% of that in the miR-control-transfected HACEs. The bars represent the means ± SEM from 4 experiments. , as compared with the miR-control. (c) The stimulatory effect of HG/thrombin on the gene expression of VCAM-1, ICAM-1, SELE, IL-6, and IL-8 significantly decreased in miR-146a mimic-transfected, HG/thrombin-stimulated HAECs to 42%, 51%, 71%, 40%, and 34% of that in miR-control-transfected, HG/thrombin-stimulated HAECs. The bars represent the means ± SEM from 3 experiments. and , as compared with the miR-control.
