5-Azacytidine Promotes an Inhibitory T-Cell Phenotype and Impairs Immune Mediated Antileukemic Activity
Figure 5
Azacytidine inhibits memory T-cells while naïve T-cell number is not affected. CD4+ and CD8+ T-cells were isolated and treated with or without the discussed 5-Aza concentrations. 48 h after treatment, cells were assessed for the expression of CD4, CD8, CD45RA, CCR7, and CD45RO. A representative sample and the gating strategy are shown in Supplementary Part (Supplementary Figure ). (a) Cells expressing both (CD45RA+/CCR7+) were considered to be naïve T-cells. (b) Cells expressing the CD45RO antigen were considered to be memory T-cells. Data were shown as mean with standard deviation, a summary of three independent experiments is shown. Plots show a percentage of gated T-cells.
