The Influence of Ouabain on Human Dendritic Cells Maturation

Figure 1

CD14 and CD1a expression patterns on monocytes cultured with Ouabain (Oua) prior to differentiation into dendritic cells. (a) Cells were incubated with medium in the presence or absence of 100 nM Oua for 24 h and then with medium or IL-4 + GM-CSF (50 ng/mL) for 5 days to allow monocyte differentiation into DC. Following this period, CD14 and CD1a expression was analyzed by flow cytometry. Representative histograms of 4 independent experiments showing CD14 and CD1a expression patterns in cells incubated only with medium, IL-4 + GM-CSF, and in cells treated previously with 100 nM Oua. R1 and R2 regions comprise positive cells for CD14 and CD1a, respectively. The percentage of positive cells and mean fluorescence intensity is indicated in each histogram. (b) Monocytes were incubated for 24 h with 100 nM Oua. Representative experiment showing CD14 expression patterns in control cells (black histogram) and in cells treated with 100 nM Ouabain for 24 h (gray histogram).

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