Research Article

Involvement of Different CD4+ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens

Figure 1

PBMCs response induced by LmES. PBMCs from HZCL and HC were stimulated with LmES (10 μg/mL) or SLA (10 μg/mL) during 5 days. (a) Proliferation was assessed by [3H]-thymidine uptake. Results were expressed as an index of proliferation (SI) (mean counts of triplicates in antigen-stimulated cultures/mean counts of triplicates in unstimulated cultures) in all tested individuals. IFN-γ (b) and GrB (c) levels were evaluated in supernatants of cultures by ELISA tests at day 5. Results were expressed as cytokine concentrations in pg/mL. Bars represent median values. Statistical analysis: , when comparing the stimulated cells to the unstimulated cells (Unst) and , when comparing HZCL to HC.
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(a)
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(b)
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(c)