Optimized “In Vitro” Culture Conditions for Human Rheumatoid Arthritis Synovial Fibroblasts

Figure 4

Effect of synovial fluid and TNFα on cytoskeleton reorganization in fibroblast-like synovial cells. RA synovial fibroblasts were cultured for 7 days in the presence of TNFα (25 ng/mL) (b), SF pool 1 (1 : 8 dilution in culture medium) (c), or medium alone (a). Actin cells were stained with Alexa Fluor 594-Phalloidin.

(a)

(b)

(c)