Troglitazone and Δ2Troglitazone Enhance Adiponectin Expression in Monocytes/Macrophages through the AMP-Activated Protein Kinase Pathway
Figure 6
TG and Δ2TG enhanced adiponectin mRNA expression was mediated through the AMPK pathway in THP-1 cells. The expression of adiponectin mRNA was examined by quantitative RT-PCR. Macrophages were treated with 150 μM of AICAR (an AMPK activator) for the indicated time (a) or with the indicated concentration for 18 h (b). Macrophages were treated with compound C (an AMPK inhibitor) for the indicated concentration and then with (c) or without (d) AICAR for 18 h and then adiponectin mRNA expression was measured by real-time PCR. Macrophages were incubated for 1 h with compound C and then for 18 h with or without 9 μM TG (e) or Δ2TG (f) in the continued presence of the inhibitor, and then, adiponectin mRNA expression was measured by real-time PCR. as compared to the untreated cells. as compared to the TG or Δ2TG-treated cells.
