Rapamycin Attenuates Endothelial Apoptosis Induced by Low Shear Stress via mTOR and Sestrin1 Related Redox Regulation
Figure 1
LSS induced EC apoptosis was attenuated by rapamycin. LSS at 2 dyne/cm2 caused EC viability reduction and apoptosis which was attenuated by rapamycin (RAPA) at the concentration of 100 ng/mL (a) HUVECs shape change and detachment after being subjected to LSS for the various time points. (b) Pretreated HUVECs with RAPA for 30 min and continuously delivered RAPA during flow study for 120 min abrogated LSS effect on cell shape change and detachment. (c) DAPI and TUNEL staining were used to label apoptotic RAECs (arrows show apoptotic cells). Adding RAPA appeased LSS induced apoptosis and RAPA alone had no influence on apoptosis. Scale bars: 100 μm ((a)-(b)), 25 μm (c).
