Imbalance between IL-17A-Producing Cells and Regulatory T Cells during Ischemic Stroke
Figure 1
Circulating IL-17A levels and RORγt mRNA expression were increased in IS patients. (a) Plasma IL-17A protein level. The plasma samples were obtained from IS patients and control subjects at 7 and 28 days after stroke and analyzed using ELISA. (b) RORγt mRNA levels in PBMCs were obtained from IS patients. The lymphocytes were isolated through the Ficoll-Hypaque separation of the peripheral blood obtained from 30 control subjects and 50 IS patients at 7 and 28 days after stroke. Total RNA was extracted from the PBMCs and the RORγt mRNA expression levels in the PBMCs obtained from various groups were analyzed using real-time PCR. All mRNA expression values were normalized to GAPDH. * and **, compared with the controls. IS-1W = 1 week after IS; IS-4W = 4 weeks after IS.
