Parasite persistence is not the necessary outcome of heart infection by T. cruzi parasites. C57BL/6 mice were infected with 103 T. cruzi blood trypomastigotes of the Y strain. At days 18 (a) and 180 (b) postinfection, mice were sacrificed, and the heart tissue was formalin-fixed, included in paraffin, and stained with hematoxylin/eosin. Arrow in the figure shows an amastigote nest, which is magnified at the insert. Bars in figures correspond to 200 μm and to 100 μm at the insert.