Lack of sensing of amastigote nests could contribute to parasite perpetuation and pathology in the chronically infected heart. Lack of sensing could occur independently of the origin of cardiomyocyte-invading trypomastigotes, that is, from a local ruptured nest or metastasis from a distant niche. The heart picture shown corresponds to a C3H/HePAS mouse infected for more than 200 days with 106 Sylvio X10/4 trypomastigotes. The tissue section was stained with hematoxylin-eosin. Bar corresponds to 100 μm (reproduced from C. R. F. Marinho, Microbes and Infection [64] with permission from Elsevier).
