Hydrogen Sulfide Ameliorates Ischemia/Reperfusion-Induced Hepatitis by Inhibiting Apoptosis and Autophagy Pathways
Figure 2
Effect of NaHS on hepatic ischemia-reperfusion injury. (a) The serum ALT and AST levels were expressed as the mean ± SD of 6 animals per group. for saline versus , for saline + I/R versus I/R + NaHS (14 μmol/kg), and for saline + I/R versus I/R + NaHS (28 μmol/kg). (b) Representative hematoxylin and eosin (H&E) stained sections of liver. Original magnifications: ×200. (c) Hepatocytes (LO2, QSG7701) that were subjected to NaHS (1 μM, 3 μM, 5 μM, 7 μM, and 9 μM), then the cells were treated with 24 h hypoxia (3% O2, 5% CO2, and 92% N2) and 2 h reoxygenation (5% CO2, 95% air). Cell viability was measured using an MTT assay and a microplate reader at a wavelength of 490 nm. The experiments were repeated three times. The results are expressed as the mean ± SD (). for (A/R + 5 μM NaHS) versus .
