Hydrogen Sulfide Ameliorates Ischemia/Reperfusion-Induced Hepatitis by Inhibiting Apoptosis and Autophagy Pathways

Figure 4

Effect of NaHS pretreatment on regulation of apoptosis in vivo. (a) The mRNA expression of Bcl-2 and Bax in liver tissues was detected by real time PCR. ^∗𝑃<0.05 for saline versus saline+I/R, ^#𝑃<0.05 for saline+I/R versus I/R + NaHS (14 μmol/kg). (b) Western blots and quantitative evaluation of the expression of Bcl-2 and Bax in liver tissues. ^∗𝑃<0.05 for saline versus saline+I/R,^#𝑃<0.05 for saline+I/R versus I/R + NaHS (14 μmol/kg). (c) Immunohistochemistry staining (200x) showed the expression of Bcl-2 and Bax in liver tissue at 12 h. ^∗𝑃<0.05 for saline+I/R versus I/R + NaHS. (d) TUNNEL staining showed the apoptotic cells in three groups at 12 h. Original magnifications: ×200. ^∗𝑃<0.05 for saline versus saline+I/R, ^#𝑃<0.05 for saline+I/R versus I/R + NaHS.

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