LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis

Figure 1

Eight-week-old mice were fed the control or the MCD diet for 8 weeks. Their livers were removed and then used for the experiments. (a) Paraffin-embedded sections were stained with hematoxylin and eosin. (b, c) RNA was isolated from the livers and mRNA levels of inflammatory cytokines (TNF-α and IL-1β) and antiapoptosis factors (IAPs and XIAP), regulated by the NF-κB transcriptional activity, were determined by real-time PCR using primers corresponding to their mRNA sequences (). The data were normalized with the expression of GAPDH mRNA. (d) Apoptotic cell death in livers was detected by TUNEL staining. The data shown are representative of three to five independent experiments and shown as means ± S.E.M. .

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