Protective Effects of N-Acetylcysteine in Concanavalin A-Induced Hepatitis in Mice

Figure 3

The effect of NAC on expression of PI3K/Akt pathway in ConA-induced autoimmune hepatitis. (a) The expression of PI3K and Akt on mRNA levels was detected by real-time PCR. Data are expressed as mean ± SD (; for ConA versus NAC 30 min; for NAC 30 min versus NAC 2 h). (b) The expressions of PI3K, p-Akt, and Akt on protein levels were detected with western blot. The expressions of PI3K, p-Akt, and Akt on protein levels of macrophages and hepatocytes were detected by western blot at 12 h time point. (c) The results of western blot were analyzed with Quantity One. Data are expressed as mean ± SD (; for ConA versus NAC 30 min; for NAC 30 min versus NAC 2 h). (d) The expressions of PI3K and p-Akt on protein levels were detected by immunohistochemistry staining in hepatic tissues at 12 h (×200 and ×400 magnification). The positive cells were indicated with red arrows. The result was analyzed using Image-Pro Plus 6.0. Date are showed as mean ± SD (; for ConA versus NAC 30 min; for NAC 30 min versus NAC 2 h).

(a)

(b)

(c)

(d)