Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging

Figure 1

Elastase signal localization and time course following Chlamydia genital tract infection. (a) Representative pseudoscaled images of mouse reproductive tracts after Elastase680 injection. All images are scaled to the same color bar with red indicating highest signal intensity and purple being the lowest. The yellow arrows point to areas of high Elastase680 signal, the uterine horns in week 1, and the ovary area in week 2. (b) Quantitation of images in part (a). Manual regions of interest (ROI) were drawn over the entire reproductive tract of mice/group and quantified as Total Radiant Efficiency (photons/s)/(μW/cm²), a sum of fluorescent pixels within the ROI. Closed symbols represent mock-challenged mice and open symbols represent naïve/challenged animals. The term naïve is used here to be consistent with terminology used later in the paper to distinguish between vaccinated and nonvaccinated (naïve) animals. ; .

(a)

(b)