Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging

Figure 2

In vivo live animal imaging with Elastase680 correlates at the oviduct level. (a) FMT data overlaid onto a uCT scan of the same mouse. The red box indicates the ROI drawn for analysis. This area correlates with the location of the ovary/oviduct which sits below the kidneys as seen in the uCT. Other areas of signal are labeled. These include the liver and bladder which are clearance organs for Elastase680. (b) Quantitation of FMT signal. Box ROIs were drawn over the ovary area of mice/group as indicated in (a) and quantified as total probe or amount of fluorescence in pmol. Closed symbols represent mock-challenged mice and open symbols represent naïve/challenged animals. (c) Quantitation of only the ovary region from Figure 1. Circular ROIs were drawn over the ovary region. The signal from each ovary was then combined for each sample and quantified as Total Radiant Efficiency. ; (d) Correlation of in vivo and ex vivo signal.

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