Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging

Table 2

Histopathology summary.

Incidence (%) and severity of histomorphologic findings in mice infected with C. muridarum
Days postinfection	7		10		14		21
Vaccination status	N	V	N	V	N	V	N	V
# Mice evaluated	15	5	5	5	25	9	15	5
Oviduct (UGT)
Inflammation-acute	60	40	80	60	96	—	7	—
Severity	++	+++	++++	+++	++++		+
Inflammation-chronic	—	—	—	—	—	11	87	40
Severity						+	++	+
Uterine horns (LGT)
Inflammation-acute	87	60	100	80	92	—	—	—
Severity	+	+	++	+	++
Inflammation-chronic	—	—	—	—	—	—	87	40
Severity							+++	++

Mice were euthanized at the indicated time point and the reproductive tracts were prepared for histopathology assessment as described in Section 2. The data presented here is a compilation of several studies. The numbers indicate the percentage of mice that had findings. Severity was graded semiquantitatively on a scale of + to +++++ according to routine procedures (+ = very slight, ++ = slight, +++ = moderate, ++++ = marked, and +++++ = severe). Acute inflammation was mostly neutrophilic while chronic inflammation was lymphocytic in nature.
N: not vaccinated; V: EB vaccinated; —: no noteworthy change; UGT: upper genital tract, and LGT: lower genital tract.