Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging

Table 3

Immunohistochemistry summary.

Incidence (%) and severity of immunohistochemical (Ly6B.2) findings in mice infected with C. muridarum
Days after challenge	7		10		14		21
Vaccination status	N	V	N	V	N	V	N	V
# Mice evaluated	15	5	5	5	25	9	15	5
Oviduct
Ly6B.2 positive	80	40	80	60	96	0	87	20
Severity	++	+++	++++	+++	++++		+	+
Uterine horns
Ly6B.2 positive	100	60	100	80	88	0	47	20
Severity	++	++	++	+	++		+	+

The same tissues as in Table 2 were also processed for immunohistochemical evaluation of neutrophils present in different regions of the reproductive tract. The numbers indicate the percentage of mice that had positive staining and the severity was a subjective assessment of the percent area of positive staining.
N: not vaccinated; V: EB vaccinated.