Sigma-1 Receptor Antagonist BD1047 Reduces Mechanical Allodynia in a Rat Model of Bone Cancer Pain through the Inhibition of Spinal NR1 Phosphorylation and Microglia Activation

Figure 2

Walker 256 cells inoculation-induced sigma-1 receptor expression increased in the spinal cord. (a) Western blot analysis showed a significant upregulation of sigma-1 protein level in the spinal cord of BCP rats on days 7, 10, 14, and 21. GAPDH was used as a loading control. (b) Quantification of sigma-1 protein level in the spinal cord. (c) Western blot analysis showed no significant change of sigma-1 protein level in the spinal cord of sham rats on days 3, 7, 10, 14, and 21. GAPDH was used as a loading control. (d) Quantification of sigma-1 protein level in the spinal cord. Sigma-1 receptor levels were normalized against GAPDH (). Results are given as means ± SEM. , versus sham group or naïve group.