Research Article
Downregulation of mPGES-1 Expression via EGR1 Plays an Important Role in Inhibition of Caffeine on PGE2 Synthesis of HBx(+) Hepatocytes
Figure 5
Effect of caffeine on EGR1 expression through PPARγ in HBx positive hepatocytes. (a) Representative immunoblots from three independent studies for PPARγ in HL7702 and HL7702-HBx cells treated with or without 800 μM caffeine for 7 days. The densitometry data were normalized to β-actin. (), statistically significant difference compared with HL7702 cells. (b) Representative immunoblots from three independent studies for PPARγ in HepG2 and HepG2-HBx cells treated with or without 800 μM caffeine for 7 days. The densitometry data were normalized to β-actin. (), statistically significant difference compared with HepG2 cells. (c) Representative immunoblots from three independent studies for EGR1 and PPARγ in HL7702 and HL7702-HBx cells treated with or without 5 mM caffeine for 6, 12, and 24 h. The densitometry data were normalized to β-actin. (), statistically significant difference compared to HL7702 cells. (d) Representative immunoblots from three independent studies for EGR1 and PPARγ in HepG2 and HepG2-HBx cells treated with or without 5 mM caffeine for 6, 12, and 24 h. The densitometry data were normalized to β-actin. (), statistically significant difference compared to HepG2 cells.
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