Differential Contribution of BLT₁ and BLT₂ to Leukotriene B₄-Induced Human NK Cell Cytotoxicity and Migration

Figure 4

Modulation of BLTR expression on human NK cells by IL-2 and IL-15. (a) NK cells were incubated for 2 or 6 h in the absence or presence of IL-2 (50 ng/mL) or IL-15 (10 ng/mL). Total RNA was then extracted and analyzed by q-PCR. Bar graphs represent mean ± SEM of five independent experiments. , by one-way ANOVA with Dunnett posttest to untreated cells (control). (b) PBLs were incubated for 18 h in the absence or presence of IL-2 or IL-15. Total expression of BLT₁ (light grey) and BLT₂ (dark grey) on NK cells was measured by FACS and expressed as fold induction relative to untreated cells. Bar graphs represent mean ± SEM of seven independent experiments. , , and by paired Student’s -test, treated versus untreated cells.

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