CNTNAP3 Associated ATG16L1 Expression and Crohn’s Disease

Figure 3

Level of ATG16L1 after CNTNAP3 manipulation. Seventy-two hours after transfection, ATG16L1 was lower in CNTNAP3 knockdown cells than that in cells transfected with empty vectors (HeLa, and SW620, ). (a and b). On the other hand, ATG16L1 was significantly higher in cells transfected with CNTNAP3 overexpression plasmid than that in cells transfected with empty vectors (HeLa, and SW620, ) (a and b). There was no significant difference between cells transfected with empty vectors and normal controls (HeLa, and SW620, ). Similarly, western blot indicated that ATG16L1 protein expression was significantly associated with the expression of CNTNAP3 manipulations. ATG16L1 protein expression significantly decreased and increased 72 hours after CNTNAP3 siRNA interference and CNTNAP3 overexpression, respectively (c and d).

(a)

(b)

(c)

(d)