5′-Adenosine Monophosphate-Induced Hypothermia Attenuates Brain Ischemia/Reperfusion Injury in a Rat Model by Inhibiting the Inflammatory Response

Figure 5

AIH decreases levels of neutrophil elastase expression in the infarcted brain. Coronal sections 24 h after operation were stained with specific antibodies: neutrophil elastase (green) and NeuN (red). The mounting medium contained DAPI, which counterstained the nuclei (blue). The merged images show the coexpression of NeuN and neutrophil elastase in yellow (a–e). Scale bar = 100 μm. (f) Neutrophil elastase-positive cells were quantitatively analyzed using ImageJ software. All values are the mean ± SEM. compared to the MCAO group, ^# compared to the NT group, and ^& compared to the HT(0.5) group.

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