Inducible Lentivirus-Mediated siRNA against TLR4 Reduces Nociception in a Rat Model of Bone Cancer Pain

Figure 1

Impact of LvOn-siTLR4 with Dox on PWT in bone cancer pain rats. The bone cancer pain was set up by the injection of Walker 256 cells into the legs of rats. After cancer cells injection, rats received intrathecal administration of the virus on the 4th day. On the 7th, 14th, and 21st days after intrathecal administration of the virus, bone cancer pain rats receiving intrathecal LvOn-siTLR4 with oral administration of doxycycline showed significantly attenuated mechanical allodynia compared to the rats treated with Lv-MM, normal saline, doxycycline, and LvOn-siTLR4 on PWT ( versus Lv-siTLR4 group, Lv-MM group, NS group, and Dox group, two-way ANOVO analysis followed by Tukey’s multiple comparisons, ).