CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway

Figure 3

siTRAF6 could significantly attenuate the agonist-CD137mAb induced increasing of TRAF6 in VSMCs. The VSMCs were treated with agonist-CD137mAb (10 mg/L) for 12 h after transfection of siTRAF6. The cells were harvested for examination of TRAF6 mRNA (a) levels by qRT-PCR and protein (b) levels by Western blot. (1: control, 2: TRAF6siRNA, 3: agonist-CD137mAb, and 4: agonist-CD137mAb + TRAF6siRNA). versus control, versus CD137; .

(a)

(b)