CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway

Figure 6

Suppressing TRAF6/NF-κB/NFATc1 significantly attenuated the agonist-CD137mAb induced increasing of IL-2 and IL-6 in VSMCs. The VSMCs were treated with agonist-CD137mAb (10 mg/L) for 12 h after transfection of siTRAF6, pretreatment with PDTC, or using a stable cell line which suppressed NFATc1. The secretion of IL-2 (a) and IL-6 (b) was measured by ELISA (PLKO.1-shNFATc1 means the gene NFATc1 is silenced by Lentivirus infection, and PLKO.1-Con means negative control) (1: control; 2: CD137; 3: CD137mAb + siTRAF6; 4: CD137 + PTDC; 5: CD137 + PLKO.1-ShNFATC1; 6: PLKO.1-control). versus control; .

(a)

(b)