The Effect of Therapeutic Blockades of Dust Particles-Induced Ca²⁺ Signaling and Proinflammatory Cytokine IL-8 in Human Bronchial Epithelial Cells

Figure 5

PM₁₀-induced Ca²⁺ signaling requires TRPM2 channel. PM₁₀ (50 μg/mL) induced Ca²⁺ signaling in the presence of TRPM2 inhibitors: (a) 75 μM 2-APB, (b) 10 μM CLZ, (c) 10 μM ACA, (d) 10 μM CLZ + 1 mM NAC, or (e) 10 μg/mL CLP. (g) TRPM2 protein was expressed in BEAS-2B cells and -actin antibody was used as loading control. Duplicated samples were loaded. Top bars on trace panels indicate the type of extracellular solutions applied to the cells. Data are represented as mean ± SE. values were considered significant. All traces are the reading from an experimental average. The dotted line shows the baseline at ratio 1.