Expression of Prostacyclin-Synthase in Human Breast Cancer: Negative Prognostic Factor and Protection against Cell Death In Vitro

Figure 1

Immunological detection of prostacyclin-synthase. (a) Western blot analysis of MCF-7 cells transfected with control vector (lane I: pCDNA 3.1), wild-type (lane II: pCDNA3.1mPGIS), and mutant prostacyclin-synthase (lane III; pCDNA3.1 mPGISC441A). As positive control PGIS from bovine aorta is shown on the left. ((b), (c)) Expression of immunoreactive PGIS in tumor cells of a ductal carcinoma showing moderate (b) or intense labeling (c). Slides were photographed at 63x magnification.

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