Expression of Prostacyclin-Synthase in Human Breast Cancer: Negative Prognostic Factor and Protection against Cell Death In Vitro

Figure 3

Effects of overexpression of PGIS and carbaprostacyclin on cell survival. (a) MCF-7 cells were transiently cotransfected with pCDNA3.1COX-2, together with control vector pCDNA3.1, pCDNA3.1mPGIS, and pCDNA3.1mPGISC441A. Cell viability upon exposure to 150 μM sulindac for 24 hours was examined by the MTT assay as compared to vehicle-treated controls (0.1% DMSO). ((b), (c)) Carbaprostacyclin-mediated protection from sulindac sulfone-induced apoptosis. CCRF-CEM cells were treated with 0, 100, and 300 μM sulindac sulfone in the presence of (b) 0.01–1 μM carbaprostacyclin or (c) 0.001–10 mM dbcAMP. 24 hours posttreatment caspase-3 activity was measured by cleavage of fluorogenic substrate Ac-DEVD-AMC.

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