Synergistic Stimulation with Different TLR7 Ligands Modulates Gene Expression Patterns in the Human Plasmacytoid Dendritic Cell Line CAL-1

Figure 4

Enhanced binding of CL264 to CAL-1 cells upon costimulation with 9.2s RNA. (a) CAL-1 cells were seeded into 24-well plates. After overnight resting, they were incubated with FITC control (5.5 µM), FITC-labeled CL264 (5 µg/mL, equaling 5.5 µM), or PLarg-complexed 9.2s RNA (2 µg/mL) or a combination for 10 minutes at +4°C. After extensive washing and fixing with PFA, cells were resuspended in FACS buffer, followed by readout on a flow cytometer. (b) Proportion of FITC-positive cells (expressed as percentage of cells incubated solely with FITC control). Combination with 9.2s RNA significantly enhances binding of FITC-CL264 to the cell surface. .

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