Synergistic Stimulation with Different TLR7 Ligands Modulates Gene Expression Patterns in the Human Plasmacytoid Dendritic Cell Line CAL-1

Table 1

Top 5 canonical pathways (signaling): costimulation for 4 hrs (CL264 and RNA).

Pathway	value
Activation of IRF by cytosolic pattern recognition receptors	<4.31
Dendritic cell maturation	<1.08
Interferon signaling	<5.94
Communication between innate and adaptive immune cells	<1.05
Death receptor signaling	<1.2

IRF: interferon regulatory factor.
CAL-1 cells were seeded into 24-well plates. After overnight resting, they were stimulated with CL264 (5 µg/mL) or 9.2s RNA (2 µg/mL) or the combination of both. After incubation for 4 hrs, cells were lysed and total RNA was extracted. Following cDNA synthesis, labeled cDNA was hybridized to microarrays. Treatment groups were normalized to untreated controls. Table lists the 5 most upregulated canonical pathways as identified by Ingenuity Pathway Analysis (IPA).