Research Article

Synergistic Stimulation with Different TLR7 Ligands Modulates Gene Expression Patterns in the Human Plasmacytoid Dendritic Cell Line CAL-1

Table 3

Genes synergistically upregulated by the combination of 9.2s RNA and CL264.

Gene nameUnigene IDGene symbolTranscript level (fold over nonstimulated control)
9.2s RNACL2649.2s RNA + CL264Synergistic increase

CD69 moleculeHs.208854CD692.46.164.27.6
BCL2-related protein A1 Hs.227817BCL2A12.722.296.63.9
baculoviral IAP repeat containing 3Hs.127799BIRC31.26.425.63.4
RALBP1 associated Eps domain containing 2Hs.186810REPS21.02.410.33.1
Interferon, beta 1, fibroblastHs.93177IFNB11.61.910.83.1
Suppressor of tumorigenicity 20Hs.729127ST201.34.818.53.0
Chemokine (C-C motif) ligand 2Hs.303649CCL20.641.4126.03.0
CD44 moleculeHs.502328CD441.54.317.53.0
Early growth response 3Hs.534313EGR32.13.315.92.9
Tubulin tyrosine ligase-like familyHs.520554TTLL21.13.111.92.8
Oligonucleotide/oligosaccharide-binding fold containing 2AHs.591610OBFC2A1.712.438.62.7
Interleukin 6 (interferon, beta 2)Hs.654458IL-60.94.914.72.6
Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alphaHs.81328NFKBIA1.66.219.62.5
MIR155 host gene (non-protein-coding) (MIR155HG), noncoding RNA.N/AMIR155HG1.51.98.12.4
Chemokine (C-C motif) ligand 1Hs.72918CCL12.415.641.82.3
RasGEF domain family, member 1BHs.591696RASGEF1B1.13.310.22.3
CD70 moleculeHs.501497CD701.46.116.52.2
G protein-coupled receptor 183Hs.784GPR1831.02.67.82.2
ADAM metallopeptidase with thrombospondin type 1 motif, 10 Hs.657508ADAMTS101.01.96.12.1
ADAM metallopeptidase domain 19Hs.483944ADAM192.43.612.62.1
FSHD region gene 2 family, member CHs.274541FRG2C0.81.14.12.1
Interleukin 23, alpha subunit p19Hs.98309IL-23A1.67.819.42.1
EF-hand calcium binding domain 3Hs.152670EFCAB31.01.34.82.1
Neutrophil cytosolic factor 2Hs.587558NCF21.83.811.52.1
Interleukin 1, betaHs.126256IL-1B1.07.317.02.1
Nuclear receptor subfamily 4, group A, member 3Hs.279522NR4A31.43.19.42.1
Tumor necrosis factor, alpha-induced protein 3Hs.211600TNFAIP31.53.510.22.1
Regulator of G-protein signaling 1Hs.75256RGS11.73.510.72.0
Serum/glucocorticoid regulated kinase 1Hs.510078SGK10.51.84.82.0
v-rel reticuloendotheliosis viral oncogene homologHs.631886REL1.12.46.72.0
Immediate early response 3Hs.76095IER30.84.39.91.9
Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zetaHs.319171NFKBIZ1.32.77.81.9
Chemokine (C-C motif) ligand 4-like 2Hs.661942CCL4L22.021.043.91.9
Interleukin 18 receptor 1Hs.469521IL-18R12.56.917.91.9
Interleukin 8 (IL8)Hs.624IL-81.82.47.91.9
CASP8 and FADD-like apoptosis regulatorHs.390736CFLAR1.52.26.81.9
pim-2 oncogeneHs.496096PIM20.83.07.11.8
Spermidine/spermine N1-acetyltransferase 1Hs.28491SAT11.63.69.51.8
Interleukin 4 induced 1Hs.574492IL-4I11.24.09.41.8
Chemokine (C-X-C motif) ligand 10Hs.632586CXCL101.65.813.11.8
Solute carrier family 29 (nucleoside transporters), member 2Hs.569017SLC29A21.43.48.61.8
Long intergenic non-protein-coding RNA 173, noncoding RNA.N/ALINC001731.83.28.81.8
Mitogen-activated protein kinase kinase kinase 8Hs.432453MAP3K81.14.19.11.8
Signal transducer and activator of transcription 5AHs.437058STAT5A0.81.94.61.7
ADP-ribosylation factor-like 5BHs.25362ARL5B1.53.17.91.7
Ninjurin 1Hs.494457NINJ10.93.47.31.7
Wingless-type MMTV integration site family, member 10AHs.121540WNT10A1.25.811.61.6
B cell translocation gene 1, antiproliferativeHs.255935BTG11.52.66.71.6
Fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)Hs.118400FSCN10.51.63.51.6
CD83 moleculeHs.595133CD831.75.211.41.6
Platelet-derived growth factor alpha polypeptideHs.535898PDGFA1.42.36.11.6
Transcription factor ECHs.125962TFEC0.63.16.11.6
CD40 molecule, TNF receptor superfamily member 5Hs.472860CD401.04.18.41.6
POU class 2 homeobox 2Hs.654420POU2F20.63.26.21.6
Nuclear receptor coactivator 7Hs.171426NCOA71.62.76.81.6
Zinc finger protein 36, C3H type-like 1Hs.85155ZFP36L11.32.05.11.6
Solute carrier family 7, member 11Hs.390594SLC7A111.42.25.71.6
FSHD region gene 2Hs.626907FRG21.11.33.71.5
Homo sapiens microRNA miR-146 stem-loopN/Ahsa-mir-146a1.13.77.31.5
Homo sapiens microRNA miR-21 stem-loopN/Ahsa-mir-211.61.75.11.5
BTB (POZ) domain containing 19Hs.632400BTBD190.81.73.91.5
ELOVL fatty acid elongase 6Hs.412939ELOVL61.11.74.21.5

CAL-1 cells were treated as described above (see Table 1). The table lists specific genes synergistically upregulated by the combination of 9.2s RNA and CL264. Genes in the costimulated group with expressions being upregulated 1.5-fold of the sum induced by the ligands 9.2s RNA and CL264 alone were defined as being synergistically regulated.