Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009

Figure 2

Effect of miRNA target insertions on the miRT-H1N1 replication in HEK293 and HBE cells. Replication of indicated viruses was assessed in HEK293 (a) and HBE (b) cells following inoculation at an MOI of 1. Virus titers were determined at 8, 16, and 24 h after inoculation on HEK293 and HBE cells. Mean virus titers and standard errors are shown. The asterisk indicates that replication of miRT-H1N1 was significantly different from that of scbl-H1N1 and from parental wt H1N1 in HBE cells (). (c) Inhibition of endogenous let-7b using anti-mir technology rescues miRT-H1N1 viral replication in the nonpermissive HBE cells. HBE cells were transfected with small oligonucleotides corresponding to the let-7b complementary sequence (anti-mir let-7b) or control nonspecific oligonucleotide (ctrl anti-mir). HBE cells were then infected with scbl-H1N1 or miRT-H1N1 virus. Viral titer values represent the mean ± SD of three independent experiments. Error bars: SD. The asterisk indicates that replication of miRT-H1N1 using ctrl anti-mir was significantly different from that of scbl-H1N1 and from miRT-H1N1 using anti-mir in HBE cells. .

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