Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009

Figure 4

Detection of apoptosis cells with flow cytometry. Cells were prepared from miRT-H1N1, scbl-H1N1, or wt H1N1-infected and uninfected HBE (a) and HEK293 (b) cells at 24 h after infection. The percentage of total apoptosis cells (late apoptotic/necrotic cells and early apoptotic cells) was shown after analysis by flow cytometry. Data were expressed as mean ± SD. . Apoptosis of cells in different conditions was shown in four-quadrant diagram. The lower left quadrant contains the normal (double negative) population, the upper left quadrant contains the dead (annexin V−/PI+) population, the upper right quadrant contains the late apoptotic/necrotic (annexin V+/PI+) cells, and the lower right quadrant contains the early apoptotic (annexin V+/PI−) cells.

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