Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009

Figure 7

Ectopic expression of let-7b inhibits the luciferase activity of reporter. (a) Schematic diagram of various sensors carrying 1 copy of MRE in the open reading frame of mRNAs. MREs that are complementary to let-7b are in italic, and mismatched parts are in bold. (b) Representative quantitative RT-PCR analysis of let-7b miRNA expression levels observed in let-7b-mimic-transfected HEK293 cells. (c) HEK293 cells were transfected with anti-mir let-7b or ctrl anti-mir. For reporter sensors, firefly luciferase (FLuc) was normalized to internal control renilla Luc (RLuc) for each sensor. The ratio of FLuc to RLuc in each group was determined. Data are mean ± SD from experiments repeated 3 times in triplicate. Error bars denote means ± SD from triplicate experiments; similar results were obtained in three independent experiments. .

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