PGE2 Elevates IL-23 Production in Human Dendritic Cells via a cAMP Dependent Pathway

Figure 4

The effect of db-cAMP and forskolin and rp-cAMP on IL-23 RNA expression of zymosan-activated Mo-DCs. Mo-DCs were pretreated for 4 h with PGE2 (2 μM) or solvent control DMSO or db-cAMP (50 μM) or forskolin (50 μM). Then the cells were incubated with zymosan (20 ug/mL) for 5 h. (a) Expression of IL-23 p19 and (b) IL-23 p40 was analyzed by RT-PCR 5 hours after stimulation. After being incubated in the presence or absence of rp-cAMP (1–125 μM) for 30 min, Mo-DCs were then treated for 4 h with PGE2 (2 μM). The cells were incubated with zymosan (20 μg/mL) for 5 h and then (c) expression of IL-23 p19 and (d) IL-23 p40 was analyzed by RT-PCR. Data represent mean ± SEM of 3 separate experiments. versus zymosan alone, versus zymosan alone, versus zymosan alone, and versus PGE2 plus zymosan treatment.

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