ATP Induces IL-1<i>β</i> Secretion in<i> Neisseria gonorrhoeae</i>-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

<div>ATP treatment does not modify caspase-1 activity in Ngo-infected MDM. The fluorescent labeled probe of caspase-1 FAM-YVAD-FMK (FAM-FITC) was incubated with uninfected MDM, gonococcus-infected MDM (Ngo+MDM), or gonococcus-infected MDM and ATP (Ngo+MDM+ATP). After washing, the fluorescent intensity retained by the MDM was determined by flow cytometry. (a) Representative histograms from one assay are shown. (b) Percentage of positive FAM cells under different conditions. Data obtained are expressed as the mean <svg height="7.35473pt" id="M15" style="vertical-align:-0.3499303pt" version="1.1" viewbox="-0.0498162 -7.0048 7.75925 7.35473" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M535 290V340H323V533H265V340H52V290H265V84H323V290H535ZM535 -22V28H52V-22H535Z" id="g117-37"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g></svg> SD and represent at least three independent experiments.</div>

Mediators of Inflammation

fig3

Figure 3

Figure 3: ATP Induces IL-1<i>β</i> Secretion in<i> Neisseria gonorrhoeae</i>-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis 

Mediators of Inflammation

ATP Induces IL-1β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

Figure 3