Irbesartan Ameliorates Diabetic Nephropathy by Suppressing the RANKL-RANK-NF-κB Pathway in Type 2 Diabetic db/db Mice

Figure 5

RANKL and RANK were overexpressed in db/db mice and downregulated by Irb. (a) Representative fields of RANKL and RANK, as labeled by immunohistochemical staining (scale bars: 50 μm). (b) Representative immunoblots of RANKL and RANK expression in the kidney. (c) Quantification of the immunohistochemical staining (A). Quantification of the immunoblot (B): the ratio between RANKL and β-actin and the ratio between RANK and β-actin are presented. The bars in panel (c) show the mean expression in arbitrary units (error bars, S.D.). compared with db/m; compared with db/db, -test.

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