CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

Figure 1

Effect of CD97 on LPS mediated TNF-α secretion in macrophages. (a) CD97 protein levels increased when monocytes differentiated into macrophages in the presence of GM-CSF (100 ng/mL). (b) LPS (0 to 60 ng/mL) treatment for 24 h inhibited CD97 protein expression in a concentration dependent manner (A). LPS (60 ng/mL) inhibited CD97 protein levels in a time gradient manner (0 to 12 h) (B). (c) Macrophages were treated with LPS (0 to 60 ng/mL) for 24 h. Cells were incubated with FITC-conjugated-anti-CD97 antibody. CD97 expression was analyzed by flow cytometry. (d) Macrophages were treated with LPS (0 to 60 ng/mL) for 24 h; total RNA was extracted and reverse transcription was performed. Finally, qRT-PCRs were performed to measure the expression of three CD97 isoforms. (e) CD97 expression/distribution in macrophages treated with or without LPS (40 ng/mL) for 24 hours by confocal microscopy. The red color represents CD97 staining. (f) siRNA of CD97 transfection upregulated TNF-α secretion whereas ectopic expression of CD97 downregulated TNF-α secretion. LPS (40 ng/mL) induced TNF-α secretion was blocked by CD97 ectopic expression ().

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