CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

Figure 4

Confocal imaging of p65 and PPAR-γ in macrophages. Macrophages were treated with LPS (40 ng/mL) or transfected with CD97 overexpression plasmid and stimulated with LPS (40 ng/mL) for 24 h. Confocal imaging of p65 and PPAR-γ was performed. When treated with LPS, p65 displayed nuclear translocation while CD97 overexpression inhibited it but strengthened PPAR-γ nuclear translocation. The green signal represented p65 and red signal depicted PPAR-γ.