Regulation of Autophagy-Related Protein and Cell Differentiation by High Mobility Group Box 1 Protein in Adipocytes

Figure 3

Autophagy was weakened by siRNA knockdown of HMGB1. HMGB1 siRNA or control siRNA was transfected into 3T3-L1 preadipocytes by lipo2000. (a) The expression levels of HMGB1, LC3, and P62 were examined by Western blot. (b–d) Quantification of protein levels normalized by GAPDH. , , and , versus the control.

(a)

(b)

(c)

(d)