Research Article
Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation and Matrix Deposition in Kidney Tissues after Injury
Table 1
qRT-PCR primers in this study.
| | Gene | Sequence (5′→3′) | GenBank accession | Product size (bp) |
| | Col1α1 | GATCCTGCCGATGTCGCTAT (F) | NM_053304.1 | 276 | | GGAGGTCTTGGTGGTTTTGTATTC (R) |
| | Col3α1 | AAGGCTGAAGGAAATAG (F) | NM_032085.1 | 147 | | AATGTCATAGGGTGCGATA (R) |
| | ZO-1 | AACAGAGCCGAGCAGTTAGCC (F) | NM_001106266.1 | 238 | | CAACATCAGCAATCGGTCCA (R) |
| | E-cadherin | GTGCCACCACCAAAGATA (F) | NM_031334.1 | 195 | | GGCTGAGACAACCCTAAT (R) |
| | α-SMA | GGCATCCACGAAACCACCT (F) | NM_031004.2 | 212 | | CCGCCGATCCAGACAGAAT (R) |
| | BMP-7 | GTGGTCAACCCTCGGCACA (F) | NM_001191856.1 | 215 | | GGCGTCTTGGAGCGATTCTG (R) |
| | MIF | TCTCCGCCACCATGCCTATG (F) | NM_031051.1 | 178 | | GGGTCGCTCGTGCCACTAAA (R) |
| | β-actin | CCCATCTATGAGGGTTACGC (F) | NM_031144.2 | 150 | | TTTAATGTCACGCACGATTTC (R) |
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