Antileishmanial Activity and Inducible Nitric Oxide Synthase Activation by RuNO Complex

Figure 1

Antileishmanial activity by ruthenium complex. (a) Amount of L. amazonensis promastigote forms after treatment with RuNO complex (30 and 60 μM) for 24 h. [Values represent the mean ± SEM of three independent experiments (). different from infected control. different between concentrations]. (b) Viability of peritoneal macrophages of BALB/c mice treated with RuNO complex (10–640 μM) for 24 h. [Values represent the mean ± SEM of three independent experiments]. (c) Percentage of infected macrophages after 24 h of incubation with RuNO complex (30 and 60 μM). (d) Mean number of amastigotes per macrophage after 24 h of incubation with RuNO complex (30 and 60 μM). (e) Recovery kinetics of promastigotes that had infected peritoneal macrophages, which were then treated with RuNO complex (30 and 60 μM). [Values represent the mean ± SEM of three independent experiments (). different from control.]

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