Antileishmanial Activity and Inducible Nitric Oxide Synthase Activation by RuNO Complex

Figure 3

(a) Nitrite levels of macrophages infected and then treated with RuNO complex (30 and 60 μM) for 24 h [ different from control, different from infected control, and different from concentrations]. (b) Determination of nitrite levels of peritoneal macrophages stimulated with LPS, infected with L. amazonensis, and then treated with 100 μM L-NAME and 60 μM RuNO complex for 24 h. [Values represent the mean ± SEM of three independent experiments (). different from control LPS and different from infected control with L-NAME]. (c) Recovery kinetics of promastigotes in peritoneal macrophages infected with L. amazonensis, treated with RuNO complex (60 μM) and the iNOS inhibitor L-NAME (100 μM) for 24 h. [ different from infected control at all times.]

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