Ethanol Extract of Sanguisorbae Radix Inhibits Mast Cell Degranulation and Suppresses 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions

Figure 5

Effects of ESR on cytotoxic and mast cell degranulation in BMMCs. (a) Cytotoxicity of BMMCs was determined using a CCK assay. BMMCs were seeded into 96-well plates and treated with various concentrations of ESR for 24 h. (b) Inhibitory effects of ESR on β-HEX release in BMMCs. BMMCs were sensitized overnight with anti-DNP IgE and challenged with DNP-HAS (Ag) with or without ESR. The data are presented as the mean ± SEM of three independent experiments. versus control.

(a)

(b)