Research Article

In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

Figure 3

Effects of 10-H2DA, 10-HDAA, and SEA on LPS-induced NO production in RAW 264.7 cells. Cells were pretreated with/without indicated concentrations of 10-H2DA, 10-HDAA, and SEA for 1 h and then stimulated with LPS (1 μg/mL) for 24 h. Control values were obtained in the absence of LPS and the three fatty acids. The values are presented as percentages of NO in comparison with LPS-treated cells. The data are the means ± SEMs for three independent experiments. Individual groups were compared by Student’s t-test (, , and compared with the LPS group).