The Regulatory Role of Rolipram on Inflammatory Mediators and Cholinergic/Adrenergic Stimulation-Induced Signals in Isolated Primary Mouse Submandibular Gland Cells

Figure 4

Effect of rolipram on synergistic regulation of cholinergic and β-adrenergic stimulation in SMG cells. Changes in induced by 10 μM carbachol (gray trace) and pretreatment with 10 μg/mL rolipram (black trace) in SMG acinar (a) and ductal (b) cells. (c) Analysis of carbachol-induced maximum peak and plateau from baseline as determined using R340/380 fluorescence ratios in acini and ductal cells. Changes in induced by 100 μM isoproterenol (gray trace) and pretreatment with 10 μg/mL rolipram (black trace) in SMG acinar (d) and ductal (e) cells. All traces were averaged. (f) Analysis of isoproterenol-induced maximum peak and plateau from baseline as determined using R340/380 fluorescence ratios in acini and ductal cells. The upper bars indicate the extracellular solutions applied to the cells.

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