Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection

Figure 4

Effect of SART1 on ISRE activity and IFN-induced JAK-STAT signaling. (a) HepG2 cells were reverse-transfected with the indicated siRNA 24 h before p-ISRE and pRL-TK cotransfection. 1000 IU/mL IFN-α was added 48 h later. Relative luminescence activity (RLA) was measured 8 h later. (b) HepG2 cells were transfected with Si-SART1 for 48 h and treated with 1000 U/mL IFN-α for 24 h (for STATs) or 1 h (for p-STATs) before cells were harvested. The expression of STAT1, STAT2, p-STAT1, and p-STAT2 was determined by western blotting. Means ± SD from three independent experiments performed in triplicate are shown. Student’s -test was used to determine significance of differences between two groups. NS = nonsignificant, , and .