Anti-Inflammatory Effects of TRAF-Interacting Protein in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

Figure 4

Overexpression of TRIP suppressed TNFα-induced NF-κB activation in RA-FLS. (a) RA-FLS-Ctrl and RA-FLS-TRIP cell lines were transfected with NF-κB reporter plasmid together with phRL-TK plasmid (internal control), 36 hours later the cells were stimulated with TNFα for 60 min, and the activation of NF-κB promoter was measured by Dual-Luciferase reporter gene assay. (b) Western blot analysis of the phosphorylation of p65 and IKKβ in RA-FLS-Ctrl or RA-FLS-TRIP group exposed to TNFα (100 ng/mL) stimulation for 60 min. , versus respective control group.

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